Information
Project
co-coating
Experiment
PATHOGEN_LABELED
PATHOGEN_TETHERED
IBIDI_OLYPMPUS
Replicate
second run
Replicate ID
1004
Errors
Settings
Assay
KE Nalm6
Plate
Custom
Cell line
un-defined
Culture medium
Substance
Evaluation interval
120 secs
Recording duration
0
Min track duration
60 secs
Movement threshold
2 µm
Time between images
60 secs
Microscopy resolution
1.5 pixel/µm
Completed on
Incubator environment:
Glossary
| Term | Unit of Measure | Definition |
| Tracks Directness | µm | Directness is a measure of the straightness of the cell track trajectory. It is calculated by comparing the Euclidean distance and the accumulated distance. A directness of 1.0 equals a straight-line migration. |
| Accumulated Distance | µm | Accumulated distance is the total distance covered by a cell whether it ran in circles or a straight line. |
| Accumulated Euclidean Distance | µm | The sum of all cells' Euclidean Distances in the well. |
| Euclidean Distance | µm/time unit | A vector of a cell track end position less the cell track start position. If a cell ends where it starts, its euclidean distance will be zero. |
| Cell Speed | µm/time unit | Speed is a change in distance divided by change in time. This requires a start and end time for the calculations. We define speed over Evaluation Interval. We define speed as a changing value over time. A cell may move fast then stop and rest. It may move in circles and end where it starts. So it may have a high speed but show no chemotaxis directionality because it ends where it starts. |
| Cell Velocity | µm/time unit | Velocity is change in distance divided by change in time where the distance is the Euclidean Distance and the time is the entire track duration. It is an indicator of chemotatic effect. |
| Center of Mass Displacement | µm | Center of Mass Displacement (CoM) is the average Euclidean Distance for the x and y axis - CoMx and CoMy correspondingly. This is a very consise indication of the average Direction and magnitude of the overall cell movement in a well or condition group. The CoM report polar chart shows the average direction and magnitude of center of mass displacement for cells in the well or condition group. The CoM bar chart shows the magnitude of the center of mass displacement average for all cells in the well or condition group. |
| CoM Displacement Magnitudes | µm | A scalar that represents the magnitude of the CoM displacements, not the direction. |
| Endpoint Tracking Chart | µm | ... |
| Evaluation Interval | time unit | Speed and accumulated distance are measured on time intervals. This time interval can be the same as the frame time, or, it can be set to a number greater than the frame time. The Frame time is an experiment setting provided by user. |
| Forward Migration Index | % | Indication of change in the center of mass (CoM) on the X and Y axes. It is the average of the difference in starting points and ending points of all cell tracks. The values are normalized by dividing the Accumulated Distance. This is useful to indicate which axes have the highest percentage of the total CoM displacement. If the chemical gradient is on the Y axis, then the FMIy should be greater to indicate chemotactic effect. |
| Mean Cell Speed Excluding Non-Moving | µm/time unit | Cell speed excluding non-moving cells. Non-moving cells are those that do not meet the thresholds set with Minimum Track Duration or Movement Threshold experiment settings. |
| Mean Cell Speed Including Non-Moving | µm/time unit | Cell speed including all cells. |
| Mean Cell Velocity Excluding Non-Moving | µm/time unit | Cell velocitiy excluding non-moving cells. Non-moving cells are those that do not meet the thresholds set with Minimum Track Duration or Movement Threshold experiment settings. |
| Mean Cell Velocity Including Non-Moving | µm/time unit | Cell velocity including all cells. |
| Mean Euclidean Distance | µm | The mean of all cells' Euclidean Distance in the well. |
| Mean Square Displacement | µm² |
This implementation of Mean Square Displacement has been borrowed from the study of the dynamics of molecules and here applied to the dynamics of cells.
The dynamics of molecules (cells) has them colliding and recolliding with each other, and observation of the trajectory
of any given molecule (cell) will find that it meanders erratically and randomly through the available volume.
Although there is no directed motion, a molecule (cell) will not remain indefinitely in the vicinity of its present position.
The question arises, "how far will a molecule (cell) travel in a given time interval?" This matter is relevant to transport
processes in the material, most notably (but not only) the rate of diffusion. The mean square displacement (msd) is a measure of the average distance a molecule (cell) travels. It is defined 
In this equation, ri(t)-ri(0) is the (vector) distance traveled by molecule (cell) i over some time interval of length t, and the squared magnitude of this vector is averaged (as indicated by the angle brackets) over many such time intervals. Often this quantity is averaged also over all molecules (cell) in the system, summing i from 1 to N and dividing by N. |
| Minimum Track Duration | time unit | Track must have a time duration greater or equal to this experiment setting to be accounted by Valid Tracks metric and to be considered a moving cell. This is an experiment setting provided by user. |
| Movement Threshold | µm | A cell must move more than this threshold to be considered as moving cell in any time interval. If the cell movement is less than this value in any time interval, cell movement will not be counted as the Cell Speed or Accumulated Distance. The value can be used to prevent noisy signals from stationary cells. This is an experiment setting provided by user. |
| Rayleigh test | Rayleigh test is a statistical test for the uniformity of a circular distribution of points (cell endpoints). With p < 0.05, the null hypothosis (uniformity) is rejected, indicating a chemotaxis effect. Like all statistical tests, this one strongly depends on the number of analyzed cells. | |
| Report Time Interval (RTI) | time unit | Dynamically calculated based on the experiment setting Time between images value and total experiment duration. Can be one of the following: minutes, seconds, hours, days. |
| Time Point | time unit | Moment in time based on setting RTI value. Starts from 0 which corresponds to the first image of the experiment recording. |
| Total Tracks | ea | The total number of tracks discovered through all time intervals. |
| Total Valid Tracks | ea | The total number of tracks discovered through all time intervals that meet the Minimum Track Duration requirement. |
| Track Length | µm | Track Length is sum of distance in each time inteval. |
| Tracks Ending In Interval | ea | The number of tracks lost at time interval. |
| Tracks In Interval | ea | The number of active cell tracks at the given time interval. If cells die or leave the field of view, this number will drop. If cells replicate or enter the field of view, this number will increase. Tracks can also be lost due to tracking errors. If this image quality is low (out of focus areas for example), this can lead to errors. If the user observes an excessive error rate, this should be reported to MetaVi Labs Support for making improvement. |
| Tracks Starting In Interval | ea | The number of new tracks obeserved in time interval. |
| Track Trajectory | Track path with a normalized origin. | |
| VisibleT cells in Interval | The number of T cells observed at each time point. Observered T cells may not be actual T cells. The cell density and aggregation can effect the AI accuracy. | |
| T Cells in Interval Excluding Short Tracks (exclude < ?? hours) | The number of T cells observed at each time point, excluding T cell tracks that had a duration less than the specified time. Observered T cells may not be actual T cells. The cell density and aggregation can effect the AI accuracy. | |
| Absolute Number Living Target Cells in Interval | The number of living target cells observed at each time point. Observered Pathogens may not be actual Pathogens. The cell density and aggregation can effect the AI accuracy. | |
| Dead or Disappeared Pathogens Cells | The total of the visible pathogens tagged as dead by AI plus pathogens that were visible in previous intervals but are now not visible (excluding those moved out of frame). | |
| Ratio of T cells to Pathogens in Interval | The number of T cells divided by the number of Pathogens in the time interval. Includes all T cell tracks existing in the interval. | |
| Absolute Number Synapses in Interval (Excluding Short Tracks) | An estimate of the number of synapses in the time interval (excludes short T cell tracks). A synapse is decudeced by observing a T cell within a 20 µm distance of a Pathogens for 30 minutes or longer. | |
| Percent of T cells in Synapse in Interval (Excluding Short Tracks) | The number of estimated T cell synapses divided by the total obeserved T cells at the time internval (excludes short T cell tracks). Because a T cell can be in close proxcimity to more than one cancer cell, it can appear to be in two synapses at once. So this measurement can be affected by high cell density. | |
| Histogram of Attacks as Ratio of Total T cells (Excluding Short Tracks) | The x-axis is the number of attacks. The y-axis is the percent of T cells that made the given number of attacks (excludes short T cell tracks). | |
| Histogram of Effective Attacks as Ratio of Total T cells (Excluding Short Tracks) | Effective attack is an attack that ended in cell death of the target. Cell death is deduced by the loss of the visible signature of a living cell, i.e. by morphological appearance. The x-axis is the number of effective attacks. The y-axis is the percent of T cells that made the given number of effective attacks (excludes short T cell tracks). | |
| Killing Efficiency (KE) |
Killing Efficiency (KE) is a metric that combines the time required to kill a population of target cells and also the ratio of effector cells to target cells.
Higher efficiency means less time and fewer effectors are required. Lower efficiency effector cells require either more time or more effectors relative to the number of targets.
The equation for KE is:
KE = (KE-Time-Factor / Killing-Duration) * (Target-To-Effector-Ratio)
Where KE-Time-Factor is set on an assay basis as 4 hours to 12 hours. This is the time basis for an ideal KE killing duration.
Killing-Duration is measured on the cytotoxicty chart to be the time from start of killing to the peak killing point.
Target-To-Effector-Ratio is the accumulated number of target cells killed by the time-point of peak killing relative to the initial effector count (at time zero).
|
Co-Culture Results Summary
View All Condition Groups Comparison Detailed Results
| Condition | Positions | Total tracks | Total valid tracks | Non-moving cells | Moving cells | Moving cells (%) |
| 10 T CELL : 1 B CELL | pos0, pos11, pos12 | 389017 | 389017 | 896 | 137211 | 99.35 |
| 5 T CELL : 1 B CELL | pos1, pos10, pos13 | 360798 | 360798 | 797 | 83743 | 99.06 |
| 2 T CELL : 1 B CELL | pos2, pos9, pos14 | 280952 | 280952 | 699 | 39885 | 98.28 |
| 1 T CELL : 1 B CELL | pos3, pos8, pos15 | 225075 | 225075 | 626 | 17842 | 96.61 |
| 1 T CELL : 5 B CELL | pos4, pos7, pos16 | 2656948 | 2656948 | 2753 | 54386 | 95.18 |
| Condition | Avg. speed, moving cells (µm/min) | Avg. speed (µm/min) | Avg. velocity, moving cells (µm/min) | Avg. velocity (µm/min) |
| 10 T CELL : 1 B CELL | 4.63 | 4.63 | 2.24 | 0.83 |
| 5 T CELL : 1 B CELL | 3.17 | 3.17 | 2.12 | 0.52 |
| 2 T CELL : 1 B CELL | 2.06 | 2.05 | 2.30 | 0.36 |
| 1 T CELL : 1 B CELL | 1.44 | 1.41 | 2.35 | 0.21 |
| 1 T CELL : 5 B CELL | 1.37 | 1.32 | 3.06 | 0.07 |
| Condition | Accumulated distance (µm) | Mean accumulated distance (µm) | Euclidean distance (µm) | Mean Euclidean distance (µm) |
| 10 T CELL : 1 B CELL | 46074112.56 | 333.32 | 10562353.39 | 27.15 |
| 5 T CELL : 1 B CELL | 20416610.82 | 241.28 | 4321268.78 | 11.98 |
| 2 T CELL : 1 B CELL | 7570851.93 | 186.38 | 1614887.41 | 5.75 |
| 1 T CELL : 1 B CELL | 3555071.28 | 192.34 | 647078.95 | 2.87 |
| 1 T CELL : 5 B CELL | 6972629.27 | 121.96 | 1088419.15 | 0.41 |
| Condition | Directness | FMIx | FMIy | CoMx (µm) | CoMy (µm) | Rayleigh test |
| 10 T CELL : 1 B CELL | 0.24 | 0.01 | 0.08 | 0.97 | 20.64 | 0.00 |
| 5 T CELL : 1 B CELL | 0.19 | 0.00 | 0.04 | 0.25 | 6.33 | 0.00 |
| 2 T CELL : 1 B CELL | 0.16 | 0.00 | 0.03 | 0.11 | 1.48 | 0.00 |
| 1 T CELL : 1 B CELL | 0.11 | 0.01 | 0.02 | 0.08 | 0.48 | 0.00 |
| 1 T CELL : 5 B CELL | 0.08 | 0.00 | 0.01 | 0.01 | 0.14 | 0.00 |
| Condition | Total Synapses | Total Synapses Resulting in Target Death | Total Synapses Not Resulting in Target Death | Effector Rate of Expansion (cells/hour) |
| 10 T CELL : 1 B CELL | 640 | 637 | 3 | -384.77 |
| 5 T CELL : 1 B CELL | 1090 | 1073 | 17 | -227.94 |
| 2 T CELL : 1 B CELL | 830 | 795 | 35 | -69.49 |
| 1 T CELL : 1 B CELL | 524 | 430 | 94 | -33.95 |
| 1 T CELL : 5 B CELL | 272 | 227 | 45 | -19.76 |
View Individual Condition Group Results
- 10 T CELL : 1 B CELL
- 5 T CELL : 1 B CELL
- 2 T CELL : 1 B CELL
- 1 T CELL : 1 B CELL
- 1 T CELL : 5 B CELL
Tracking data:
Total tracks
389017
FMIx
0.01
FMIy
0.08
CoMx
0.97 µm
CoMy
20.64 µm
Avg. speed, moving cells
4.63 µm/min
Avg. speed
4.63 µm/min
Avg. velocity, moving cells
2.24 µm/min
Avg. velocity
0.83 µm/min
Directness
0.24
Accumulated distance
46074112.56 µm
Euclidean distance
10562353.39 µm
Rayleigh test
0.00
Tracking data:
Total tracks
360798
FMIx
0.00
FMIy
0.04
CoMx
0.25 µm
CoMy
6.33 µm
Avg. speed, moving cells
3.17 µm/min
Avg. speed
3.17 µm/min
Avg. velocity, moving cells
2.12 µm/min
Avg. velocity
0.52 µm/min
Directness
0.19
Accumulated distance
20416610.82 µm
Euclidean distance
4321268.78 µm
Rayleigh test
0.00
Tracking data:
Total tracks
280952
FMIx
0.00
FMIy
0.03
CoMx
0.11 µm
CoMy
1.48 µm
Avg. speed, moving cells
2.06 µm/min
Avg. speed
2.05 µm/min
Avg. velocity, moving cells
2.30 µm/min
Avg. velocity
0.36 µm/min
Directness
0.16
Accumulated distance
7570851.93 µm
Euclidean distance
1614887.41 µm
Rayleigh test
0.00
Tracking data:
Total tracks
225075
FMIx
0.01
FMIy
0.02
CoMx
0.08 µm
CoMy
0.48 µm
Avg. speed, moving cells
1.44 µm/min
Avg. speed
1.41 µm/min
Avg. velocity, moving cells
2.35 µm/min
Avg. velocity
0.21 µm/min
Directness
0.11
Accumulated distance
3555071.28 µm
Euclidean distance
647078.95 µm
Rayleigh test
0.00
Tracking data:
Total tracks
2656948
FMIx
0.00
FMIy
0.01
CoMx
0.01 µm
CoMy
0.14 µm
Avg. speed, moving cells
1.37 µm/min
Avg. speed
1.32 µm/min
Avg. velocity, moving cells
3.06 µm/min
Avg. velocity
0.07 µm/min
Directness
0.08
Accumulated distance
6972629.27 µm
Euclidean distance
1088419.15 µm
Rayleigh test
0.00
View Wells Comparison Results
Tabular Results, all wells:
| Well | Total tracks | Total valid tracks | Non-moving cells | Moving cells | Moving cells (%) |
| pos0 | 136130 | 136130 | 255 | 50945 | 99.50 |
| pos1 | 134778 | 134778 | 293 | 30821 | 99.06 |
| pos2 | 82679 | 82679 | 205 | 11432 | 98.24 |
| pos3 | 78524 | 78524 | 221 | 6962 | 96.92 |
| pos4 | 730004 | 730004 | 1155 | 15678 | 93.14 |
| pos7 | 758178 | 758178 | 801 | 18376 | 95.82 |
| pos8 | 76494 | 76494 | 197 | 4522 | 95.83 |
| pos9 | 100684 | 100684 | 230 | 13821 | 98.36 |
| pos10 | 102787 | 102787 | 241 | 24152 | 99.01 |
| pos11 | 123731 | 123731 | 416 | 41412 | 99.01 |
| pos12 | 129156 | 129156 | 252 | 44985 | 99.44 |
| pos13 | 123233 | 123233 | 270 | 28842 | 99.07 |
| pos14 | 97589 | 97589 | 267 | 14673 | 98.21 |
| pos15 | 70057 | 70057 | 215 | 6377 | 96.74 |
| pos16 | 1168766 | 1168766 | 804 | 20393 | 96.21 |
Tabular Results, all wells:
| Well | Avg. speed, moving cells (µm/min) | Avg. speed (µm/min) | Avg. velocity, moving cells (µm/min) | Avg. velocity (µm/min) |
| pos0 | 4.85 | 4.85 | 2.31 | 0.91 |
| pos1 | 3.04 | 3.04 | 2.10 | 0.51 |
| pos2 | 1.93 | 1.92 | 2.29 | 0.34 |
| pos3 | 1.52 | 1.50 | 2.35 | 0.23 |
| pos4 | 1.23 | 1.18 | 2.85 | 0.07 |
| pos7 | 1.34 | 1.29 | 2.95 | 0.08 |
| pos8 | 1.26 | 1.22 | 2.28 | 0.15 |
| pos9 | 2.03 | 2.02 | 2.31 | 0.35 |
| pos10 | 3.23 | 3.23 | 2.13 | 0.53 |
| pos11 | 4.34 | 4.34 | 2.12 | 0.75 |
| pos12 | 4.61 | 4.61 | 2.26 | 0.83 |
| pos13 | 3.25 | 3.25 | 2.14 | 0.53 |
| pos14 | 2.21 | 2.20 | 2.31 | 0.38 |
| pos15 | 1.53 | 1.50 | 2.39 | 0.24 |
| pos16 | 1.52 | 1.46 | 3.32 | 0.07 |
Tabular Results, all wells:
| Well | Accumulated distance (µm) | Mean accumulated distance (µm) | Euclidean distance (µm) | Mean Euclidean distance (µm) |
| pos0 | 18443523.97 | 360.23 | 4511984.31 | 33.14 |
| pos1 | 7037455.32 | 226.18 | 1526523.37 | 11.33 |
| pos2 | 2220883.16 | 190.85 | 465035.68 | 5.62 |
| pos3 | 1345856.14 | 187.37 | 262196.84 | 3.34 |
| pos4 | 1942396.49 | 115.39 | 276333.70 | 0.38 |
| pos7 | 2417879.38 | 126.08 | 365116.53 | 0.48 |
| pos8 | 947276.92 | 200.74 | 155333.66 | 2.03 |
| pos9 | 2510348.67 | 178.66 | 542536.16 | 5.39 |
| pos10 | 6108966.50 | 250.44 | 1265935.14 | 12.32 |
| pos11 | 12222889.09 | 292.22 | 2534210.98 | 20.48 |
| pos12 | 15407699.50 | 340.60 | 3516158.10 | 27.22 |
| pos13 | 7270189.00 | 249.73 | 1528810.27 | 12.41 |
| pos14 | 2839620.10 | 190.07 | 607315.57 | 6.22 |
| pos15 | 1261938.22 | 191.43 | 229548.45 | 3.28 |
| pos16 | 2612353.40 | 123.24 | 446968.92 | 0.38 |
Tabular Results, all wells:
| Well | Directness | FMIx | FMIy | CoMx (µm) | CoMy (µm) | Rayleigh test |
| pos0 | 0.25 | 0.01 | 0.09 | 1.01 | 27.20 | 0.00 |
| pos1 | 0.20 | 0.01 | 0.05 | 0.42 | 6.15 | 0.00 |
| pos2 | 0.15 | 0.01 | 0.03 | 0.19 | 1.23 | 0.00 |
| pos3 | 0.14 | 0.03 | 0.02 | 0.00 | 0.65 | 0.00 |
| pos4 | 0.07 | 0.01 | 0.01 | 0.02 | 0.11 | 0.00 |
| pos7 | 0.09 | 0.00 | 0.02 | 0.02 | 0.16 | 0.00 |
| pos8 | 0.08 | 0.00 | 0.00 | 0.31 | 0.26 | 0.00 |
| pos9 | 0.16 | 0.00 | 0.03 | 0.04 | 1.46 | 0.00 |
| pos10 | 0.17 | 0.00 | 0.03 | 0.00 | 5.86 | 0.00 |
| pos11 | 0.23 | 0.01 | 0.06 | 1.28 | 13.01 | 0.00 |
| pos12 | 0.24 | 0.01 | 0.08 | 0.62 | 21.03 | 0.00 |
| pos13 | 0.18 | 0.00 | 0.04 | 0.27 | 6.93 | 0.00 |
| pos14 | 0.16 | 0.00 | 0.02 | 0.11 | 1.70 | 0.00 |
| pos15 | 0.11 | 0.00 | 0.03 | -0.07 | 0.54 | 0.00 |
| pos16 | 0.07 | 0.00 | 0.01 | 0.00 | 0.14 | 0.00 |
| Well | Total Synapses | Total Synapses Resulting in Target Death | Total Synapses Not Resulting in Target Death | Effector Rate of Expansion (cells/hour) |
| pos0 | 716 | 710 | 6 | -2804.44 |
| pos1 | 1460 | 1425 | 35 | -2558.33 |
| pos2 | 1181 | 1065 | 116 | -1974.55 |
| pos3 | 854 | 726 | 128 | -870.00 |
| pos4 | 1036 | 865 | 171 | -2940.00 |
| pos7 | 1306 | 1166 | 140 | -2676.67 |
| pos8 | 621 | 546 | 75 | -1590.00 |
| pos9 | 1315 | 1256 | 59 | -1432.94 |
| pos10 | 1195 | 1183 | 12 | -1691.74 |
| pos11 | 625 | 625 | 0 | -911.13 |
| pos12 | 848 | 844 | 4 | -1704.75 |
| pos13 | 1308 | 1257 | 51 | -677.27 |
| pos14 | 1309 | 1211 | 98 | -1871.54 |
| pos15 | 829 | 697 | 132 | -422.65 |
| pos16 | 925 | 842 | 83 | -2157.27 |
View Individual Well Results
pos0
Well tracking video
Tracking data:
Total tracks
136130
FMIx
0.01
FMIy
0.09
CoMx
1.01 µm
CoMy
27.20 µm
Avg. speed, moving cells
4.85 µm/min
Avg. speed
4.85 µm/min
Avg. velocity, moving cells
2.31 µm/min
Avg. velocity
0.91 µm/min
Directness
0.25
Accumulated distance
18443523.97 µm
Euclidean distance
4511984.31 µm
Rayleigh test
0.00
pos1
Well tracking video
Tracking data:
Total tracks
134778
FMIx
0.01
FMIy
0.05
CoMx
0.42 µm
CoMy
6.15 µm
Avg. speed, moving cells
3.04 µm/min
Avg. speed
3.04 µm/min
Avg. velocity, moving cells
2.10 µm/min
Avg. velocity
0.51 µm/min
Directness
0.20
Accumulated distance
7037455.32 µm
Euclidean distance
1526523.37 µm
Rayleigh test
0.00
pos2
Well tracking video
Tracking data:
Total tracks
82679
FMIx
0.01
FMIy
0.03
CoMx
0.19 µm
CoMy
1.23 µm
Avg. speed, moving cells
1.93 µm/min
Avg. speed
1.92 µm/min
Avg. velocity, moving cells
2.29 µm/min
Avg. velocity
0.34 µm/min
Directness
0.15
Accumulated distance
2220883.16 µm
Euclidean distance
465035.68 µm
Rayleigh test
0.00
pos3
Well tracking video
Tracking data:
Total tracks
78524
FMIx
0.03
FMIy
0.02
CoMx
0.00 µm
CoMy
0.65 µm
Avg. speed, moving cells
1.52 µm/min
Avg. speed
1.50 µm/min
Avg. velocity, moving cells
2.35 µm/min
Avg. velocity
0.23 µm/min
Directness
0.14
Accumulated distance
1345856.14 µm
Euclidean distance
262196.84 µm
Rayleigh test
0.00
pos4
Well tracking video
Tracking data:
Total tracks
730004
FMIx
0.01
FMIy
0.01
CoMx
0.02 µm
CoMy
0.11 µm
Avg. speed, moving cells
1.23 µm/min
Avg. speed
1.18 µm/min
Avg. velocity, moving cells
2.85 µm/min
Avg. velocity
0.07 µm/min
Directness
0.07
Accumulated distance
1942396.49 µm
Euclidean distance
276333.70 µm
Rayleigh test
0.00
pos7
Well tracking video
Tracking data:
Total tracks
758178
FMIx
0.00
FMIy
0.02
CoMx
0.02 µm
CoMy
0.16 µm
Avg. speed, moving cells
1.34 µm/min
Avg. speed
1.29 µm/min
Avg. velocity, moving cells
2.95 µm/min
Avg. velocity
0.08 µm/min
Directness
0.09
Accumulated distance
2417879.38 µm
Euclidean distance
365116.53 µm
Rayleigh test
0.00
pos8
Well tracking video
Tracking data:
Total tracks
76494
FMIx
0.00
FMIy
0.00
CoMx
0.31 µm
CoMy
0.26 µm
Avg. speed, moving cells
1.26 µm/min
Avg. speed
1.22 µm/min
Avg. velocity, moving cells
2.28 µm/min
Avg. velocity
0.15 µm/min
Directness
0.08
Accumulated distance
947276.92 µm
Euclidean distance
155333.66 µm
Rayleigh test
0.00
pos9
Well tracking video
Tracking data:
Total tracks
100684
FMIx
0.00
FMIy
0.03
CoMx
0.04 µm
CoMy
1.46 µm
Avg. speed, moving cells
2.03 µm/min
Avg. speed
2.02 µm/min
Avg. velocity, moving cells
2.31 µm/min
Avg. velocity
0.35 µm/min
Directness
0.16
Accumulated distance
2510348.67 µm
Euclidean distance
542536.16 µm
Rayleigh test
0.00
pos10
Well tracking video
Tracking data:
Total tracks
102787
FMIx
0.00
FMIy
0.03
CoMx
0.00 µm
CoMy
5.86 µm
Avg. speed, moving cells
3.23 µm/min
Avg. speed
3.23 µm/min
Avg. velocity, moving cells
2.13 µm/min
Avg. velocity
0.53 µm/min
Directness
0.17
Accumulated distance
6108966.50 µm
Euclidean distance
1265935.14 µm
Rayleigh test
0.00
pos11
Well tracking video
Tracking data:
Total tracks
123731
FMIx
0.01
FMIy
0.06
CoMx
1.28 µm
CoMy
13.01 µm
Avg. speed, moving cells
4.34 µm/min
Avg. speed
4.34 µm/min
Avg. velocity, moving cells
2.12 µm/min
Avg. velocity
0.75 µm/min
Directness
0.23
Accumulated distance
12222889.09 µm
Euclidean distance
2534210.98 µm
Rayleigh test
0.00
pos12
Well tracking video
Tracking data:
Total tracks
129156
FMIx
0.01
FMIy
0.08
CoMx
0.62 µm
CoMy
21.03 µm
Avg. speed, moving cells
4.61 µm/min
Avg. speed
4.61 µm/min
Avg. velocity, moving cells
2.26 µm/min
Avg. velocity
0.83 µm/min
Directness
0.24
Accumulated distance
15407699.50 µm
Euclidean distance
3516158.10 µm
Rayleigh test
0.00
pos13
Well tracking video
Tracking data:
Total tracks
123233
FMIx
0.00
FMIy
0.04
CoMx
0.27 µm
CoMy
6.93 µm
Avg. speed, moving cells
3.25 µm/min
Avg. speed
3.25 µm/min
Avg. velocity, moving cells
2.14 µm/min
Avg. velocity
0.53 µm/min
Directness
0.18
Accumulated distance
7270189.00 µm
Euclidean distance
1528810.27 µm
Rayleigh test
0.00
pos14
Well tracking video
Tracking data:
Total tracks
97589
FMIx
0.00
FMIy
0.02
CoMx
0.11 µm
CoMy
1.70 µm
Avg. speed, moving cells
2.21 µm/min
Avg. speed
2.20 µm/min
Avg. velocity, moving cells
2.31 µm/min
Avg. velocity
0.38 µm/min
Directness
0.16
Accumulated distance
2839620.10 µm
Euclidean distance
607315.57 µm
Rayleigh test
0.00
pos15
Well tracking video
Tracking data:
Total tracks
70057
FMIx
0.00
FMIy
0.03
CoMx
-0.07 µm
CoMy
0.54 µm
Avg. speed, moving cells
1.53 µm/min
Avg. speed
1.50 µm/min
Avg. velocity, moving cells
2.39 µm/min
Avg. velocity
0.24 µm/min
Directness
0.11
Accumulated distance
1261938.22 µm
Euclidean distance
229548.45 µm
Rayleigh test
0.00
pos16
Well tracking video
Tracking data:
Total tracks
1168766
FMIx
0.00
FMIy
0.01
CoMx
0.00 µm
CoMy
0.14 µm
Avg. speed, moving cells
1.52 µm/min
Avg. speed
1.46 µm/min
Avg. velocity, moving cells
3.32 µm/min
Avg. velocity
0.07 µm/min
Directness
0.07
Accumulated distance
2612353.40 µm
Euclidean distance
446968.92 µm
Rayleigh test
0.00