Information
Project
wound healing demo
Experiment
wound healing demo
Replicate
Replicate ID
9
Errors
Settings
Assay
Wound Healing
Plate
Custom
Cell line
un-defined
Culture medium
Substance
Evaluation interval
120 secs
Recording duration
0
Min track duration
10 secs
Movement threshold
2 µm
Time between images
600 secs
Microscopy resolution
1 pixel/µm
Completed on
Incubator environment:
Glossary
| Term | Unit of Measure | Definition |
| Analyzed Area Margin | µm² | The red outside line on Pic 1 and Pic 2 indicates the margin. Outside the margin algorithms cannot reliably detect structures, so this region is omitted from the analysis. The width of the margin is dependent on the resolution. The higher the resolution the smaller the margin. |
| Initial Scratch Region | µm² | Area between vertical green lines on Pic 1 and Pic 2, which is 10% wider on each side of the scratch, increasing scratch analysis area. |
| Report Time Interval (RTI) | time unit | Dynamically calculated based on the experiment setting Time between images value and total experiment duration. Can be one of the following: minutes, seconds, hours, days. |
| Scratch Closure Speed, Linear Fit Interval | µm²/RTI | Speed at which scratch not covered by cells (open) area is closing during the experiment in the interval where scratch open area is closing with consistent pace fitting linear filter. |
| Scratch Closure Speed, Zero-to-Peak Interval | µm²/RTI | Speed at which scratch open area is closing during the experiment starting from Time point 0 to time point with maximum value of Scratch Covered Area. |
| Scratch Covered Area | µm² | Area within green lines on Pic 1 and Pic 2 covered by cells at given time point. |
| Scratch Covered Area % | % | Percentage covered by cells scratch area (Starting Scratch Open Area - Scratch Open Area) of the total Starting Scratch Open Area at given time point. |
| Scratch Open Area | µm² | Area within green lines on Pic 1 and Pic 2 not covered by cells (open) at given time point. |
| Scratch Open Area % | % | Percentage uncovered scratch area (100% - Scratch Covered Area %) |
| Starting Scratch Covered Area | µm² | Area marked blue on Pic 2 at Time point 0. |
| Starting Scratch Open Area | µm² | Area marked white on Pic 2 at Time point 0. |
| Time Point | time unit | Moment in time based on setting RTI value. Starts from 0 which corresponds to the first image of the experiment recording. |
| Total Area | µm² | Area within Analyzed Area Margin at given time point. |
| Total Covered Area | µm² | Area within Analyzed Area Margin covered by cells at given time point. |
| Total Open Area | µm² | Area within Analyzed Area Margin not covered by cells (open) at given time point. |
|
|
View Wells Comparison Results
View Individual Well Results
C1
Well tracking video
Well images over time
Well over time:
| Time Point (hh:mm) | Total Area (µm²) | Total Covered Area (µm²) | Total Open Area (µm²) | Scratch Open Area (%) | Scratch Covered Area (%) |
| 00:00 | 1.32738E+06 | 802972 | 524403 | 100 | 0 |
| 00:10 | 1.32738E+06 | 862899 | 464476 | 85.2981 | 14.7019 |
| 00:20 | 1.32738E+06 | 900425 | 426950 | 78.1876 | 21.8124 |
| 00:30 | 1.32738E+06 | 929888 | 397487 | 72.5114 | 27.4886 |
| 00:40 | 1.32738E+06 | 972027 | 355348 | 64.7049 | 35.2951 |
| 00:50 | 1.32738E+06 | 1.01365E+06 | 313726 | 57.375 | 42.625 |
| 01:00 | 1.32738E+06 | 1.04705E+06 | 280328 | 50.631 | 49.3691 |
| 01:10 | 1.32738E+06 | 1.06427E+06 | 263105 | 47.0533 | 52.9467 |
| 01:20 | 1.32738E+06 | 1.09034E+06 | 237036 | 43.1708 | 56.8292 |
| 01:30 | 1.32738E+06 | 1.11672E+06 | 210660 | 38.464 | 61.536 |
| 01:40 | 1.32738E+06 | 1.13455E+06 | 192823 | 35.0478 | 64.9522 |
| 01:50 | 1.32738E+06 | 1.15527E+06 | 172103 | 30.9292 | 69.0708 |
| 02:00 | 1.32738E+06 | 1.1414E+06 | 185977 | 33.0274 | 66.9726 |
| 02:10 | 1.32738E+06 | 1.17194E+06 | 155433 | 27.8943 | 72.1057 |
| 02:20 | 1.32738E+06 | 1.20106E+06 | 126314 | 22.5407 | 77.4593 |
| 02:30 | 1.32738E+06 | 1.22151E+06 | 105867 | 19.516 | 80.484 |
| 02:40 | 1.32738E+06 | 1.23529E+06 | 92086 | 17.0591 | 82.9409 |
| 02:50 | 1.32738E+06 | 1.23998E+06 | 87396 | 16.1392 | 83.8609 |
| 03:00 | 1.32738E+06 | 1.25493E+06 | 72443 | 13.1162 | 86.8838 |
| 03:10 | 1.32738E+06 | 1.26926E+06 | 58113 | 10.5755 | 89.4246 |
| 03:20 | 1.32738E+06 | 1.27369E+06 | 53688 | 9.15424 | 90.8458 |
| 03:30 | 1.32738E+06 | 1.27784E+06 | 49535 | 7.17527 | 92.8247 |
| 03:40 | 1.32738E+06 | 1.29387E+06 | 33502 | 5.36005 | 94.64 |
| 03:50 | 1.32738E+06 | 1.30259E+06 | 24786 | 3.69101 | 96.309 |
| 04:00 | 1.32738E+06 | 1.31017E+06 | 17201 | 2.61407 | 97.3859 |
| 04:10 | 1.32738E+06 | 1.30943E+06 | 17944 | 2.78359 | 97.2164 |
| 04:20 | 1.32738E+06 | 1.31052E+06 | 16858 | 2.35254 | 97.6475 |
| 04:30 | 1.32738E+06 | 1.30959E+06 | 17785 | 2.23258 | 97.7674 |
| 04:40 | 1.32738E+06 | 1.30826E+06 | 19112 | 2.46055 | 97.5395 |
| 04:50 | 1.32738E+06 | 1.30962E+06 | 17757 | 1.64551 | 98.3545 |
| 05:00 | 1.32738E+06 | 1.31512E+06 | 12259 | 1.19228 | 98.8077 |
| 05:10 | 1.32738E+06 | 1.31156E+06 | 15814 | 1.50993 | 98.4901 |
| 05:20 | 1.32738E+06 | 1.30974E+06 | 17634 | 2.21523 | 97.7848 |
| 05:30 | 1.32738E+06 | 1.31212E+06 | 15254 | 1.89488 | 98.1051 |
| 05:40 | 1.32738E+06 | 1.29783E+06 | 29549 | 3.05225 | 96.9478 |
| 05:50 | 1.32738E+06 | 1.30941E+06 | 17965 | 1.82333 | 98.1767 |
| 06:00 | 1.32738E+06 | 1.30537E+06 | 22010 | 3.02486 | 96.9751 |
| 06:10 | 1.32738E+06 | 1.3147E+06 | 12671 | 0.932297 | 99.0677 |
| 06:20 | 1.32738E+06 | 1.30991E+06 | 17466 | 1.70529 | 98.2947 |
| 06:30 | 1.32738E+06 | 1.30731E+06 | 20067 | 2.14695 | 97.8531 |
| 06:40 | 1.32738E+06 | 1.30323E+06 | 24148 | 2.88407 | 97.1159 |
| 06:50 | 1.32738E+06 | 1.30213E+06 | 25241 | 3.61984 | 96.3802 |
| 07:00 | 1.32738E+06 | 1.31229E+06 | 15088 | 1.97376 | 98.0262 |
| 07:10 | 1.32738E+06 | 1.29817E+06 | 29208 | 4.21753 | 95.7825 |
| 07:20 | 1.32738E+06 | 1.30934E+06 | 18034 | 2.63181 | 97.3682 |
| 07:30 | 1.32738E+06 | 1.31352E+06 | 13853 | 2.0997 | 97.9003 |
C2
Well tracking video
Well images over time
Well over time:
| Time Point (hh:mm) | Total Area (µm²) | Total Covered Area (µm²) | Total Open Area (µm²) | Scratch Open Area (%) | Scratch Covered Area (%) |
| 00:00 | 1.32738E+06 | 537260 | 790115 | 100 | 0 |
| 00:10 | 1.32738E+06 | 551297 | 776078 | 97.9498 | 2.05023 |
| 00:20 | 1.32738E+06 | 568842 | 758533 | 95.8426 | 4.15745 |
| 00:30 | 1.32738E+06 | 590388 | 736987 | 93.0903 | 6.90971 |
| 00:40 | 1.32738E+06 | 616222 | 711153 | 89.9003 | 10.0997 |
| 00:50 | 1.32738E+06 | 642227 | 685148 | 86.6195 | 13.3805 |
| 01:00 | 1.32738E+06 | 670882 | 656493 | 82.9698 | 17.0302 |
| 01:10 | 1.32738E+06 | 699668 | 627707 | 79.3172 | 20.6828 |
| 01:20 | 1.32738E+06 | 728864 | 598511 | 75.6177 | 24.3823 |
| 01:30 | 1.32738E+06 | 753936 | 573439 | 72.4526 | 27.5474 |
| 01:40 | 1.32738E+06 | 786113 | 541262 | 68.3624 | 31.6376 |
| 01:50 | 1.32738E+06 | 810710 | 516665 | 65.2609 | 34.7391 |
| 02:00 | 1.32738E+06 | 834781 | 492594 | 62.1444 | 37.8556 |
| 02:10 | 1.32738E+06 | 856826 | 470549 | 59.3594 | 40.6406 |
| 02:20 | 1.32738E+06 | 877886 | 449489 | 56.7173 | 43.2827 |
| 02:30 | 1.32738E+06 | 900879 | 426496 | 53.8365 | 46.1635 |
| 02:40 | 1.32738E+06 | 920430 | 406945 | 51.3664 | 48.6336 |
| 02:50 | 1.32738E+06 | 938416 | 388959 | 49.09 | 50.91 |
| 03:00 | 1.32738E+06 | 951173 | 376202 | 47.4856 | 52.5144 |
| 03:10 | 1.32738E+06 | 972284 | 355091 | 44.8006 | 55.1994 |
| 03:20 | 1.32738E+06 | 988967 | 338408 | 42.6993 | 57.3007 |
| 03:30 | 1.32738E+06 | 999799 | 327576 | 41.3274 | 58.6726 |
| 03:40 | 1.32738E+06 | 1.03013E+06 | 297249 | 37.4547 | 62.5454 |
| 03:50 | 1.32738E+06 | 1.05287E+06 | 274510 | 34.6381 | 65.3619 |
| 04:00 | 1.32738E+06 | 1.06813E+06 | 259247 | 32.7069 | 67.2931 |
| 04:10 | 1.32738E+06 | 1.0834E+06 | 243973 | 30.8191 | 69.1809 |
| 04:20 | 1.32738E+06 | 1.10351E+06 | 223868 | 28.2843 | 71.7157 |
| 04:30 | 1.32738E+06 | 1.1226E+06 | 204780 | 25.8286 | 74.1714 |
| 04:40 | 1.32738E+06 | 1.13372E+06 | 193656 | 24.4614 | 75.5386 |
| 04:50 | 1.32738E+06 | 1.15695E+06 | 170428 | 21.528 | 78.472 |
| 05:00 | 1.32738E+06 | 1.17842E+06 | 148957 | 18.7896 | 81.2104 |
| 05:10 | 1.32738E+06 | 1.19169E+06 | 135684 | 17.1224 | 82.8776 |
| 05:20 | 1.32738E+06 | 1.23226E+06 | 95118 | 12.004 | 87.9961 |
| 05:30 | 1.32738E+06 | 1.25296E+06 | 74418 | 9.38326 | 90.6167 |
| 05:40 | 1.32738E+06 | 1.27036E+06 | 57020 | 7.18955 | 92.8105 |
| 05:50 | 1.32738E+06 | 1.28975E+06 | 37630 | 4.72943 | 95.2706 |
| 06:00 | 1.32738E+06 | 1.3051E+06 | 22272 | 2.79101 | 97.209 |
| 06:10 | 1.32738E+06 | 1.31134E+06 | 16036 | 2.0163 | 97.9837 |
| 06:20 | 1.32738E+06 | 1.31922E+06 | 8160 | 1.0267 | 98.9733 |
| 06:30 | 1.32738E+06 | 1.32344E+06 | 3940 | 0.489044 | 99.511 |
| 06:40 | 1.32738E+06 | 1.3216E+06 | 5771 | 0.723427 | 99.2766 |
| 06:50 | 1.32738E+06 | 1.31918E+06 | 8198 | 1.00024 | 98.9998 |
| 07:00 | 1.32738E+06 | 1.32125E+06 | 6128 | 0.775971 | 99.224 |
| 07:10 | 1.32738E+06 | 1.32398E+06 | 3397 | 0.430153 | 99.5699 |
| 07:20 | 1.32738E+06 | 1.32554E+06 | 1831 | 0.203613 | 99.7964 |
C3
Well tracking video
Well images over time
Well over time:
| Time Point (hh:mm) | Total Area (µm²) | Total Covered Area (µm²) | Total Open Area (µm²) | Scratch Open Area (%) | Scratch Covered Area (%) |
| 00:00 | 3.11966E+06 | 1.02083E+06 | 2.09883E+06 | 100 | 0 |
| 00:10 | 3.11966E+06 | 1.13722E+06 | 1.98244E+06 | 94.3831 | 5.61686 |
| 00:20 | 3.11966E+06 | 1.24665E+06 | 1.87301E+06 | 88.8378 | 11.1622 |
| 00:30 | 3.11966E+06 | 1.34228E+06 | 1.77738E+06 | 83.8455 | 16.1545 |
| 00:40 | 3.11966E+06 | 1.47528E+06 | 1.64439E+06 | 77.8644 | 22.1356 |
| 00:50 | 3.11966E+06 | 1.61805E+06 | 1.50161E+06 | 70.9246 | 29.0754 |
| 01:00 | 3.11966E+06 | 1.7696E+06 | 1.35007E+06 | 63.8393 | 36.1607 |
| 01:10 | 3.11966E+06 | 1.93405E+06 | 1.18561E+06 | 56.2616 | 43.7384 |
| 01:20 | 3.11966E+06 | 2.07997E+06 | 1.03969E+06 | 49.1806 | 50.8194 |
| 01:30 | 3.11966E+06 | 2.22955E+06 | 890115 | 42.0809 | 57.9191 |
| 01:40 | 3.11966E+06 | 2.35178E+06 | 767883 | 36.0302 | 63.9698 |
| 01:50 | 3.11966E+06 | 2.45711E+06 | 662548 | 30.7301 | 69.2699 |
| 02:00 | 3.11966E+06 | 2.60716E+06 | 512504 | 24.2661 | 75.7339 |
| 02:10 | 3.11966E+06 | 2.73789E+06 | 381775 | 17.8397 | 82.1603 |
| 02:20 | 3.11966E+06 | 2.8861E+06 | 233563 | 10.9747 | 89.0253 |
| 02:30 | 3.11966E+06 | 2.99389E+06 | 125768 | 5.96513 | 94.0349 |
| 02:40 | 3.11966E+06 | 3.07055E+06 | 49116 | 2.23485 | 97.7652 |
| 02:50 | 3.11966E+06 | 3.05365E+06 | 66012 | 2.90436 | 97.0956 |
| 03:00 | 3.11966E+06 | 3.09493E+06 | 24732 | 1.12596 | 98.874 |
| 03:10 | 3.11966E+06 | 3.10205E+06 | 17610 | 0.831261 | 99.1687 |
| 03:20 | 3.11966E+06 | 3.11505E+06 | 4611 | 0.153923 | 99.8461 |
| 03:30 | 3.11966E+06 | 3.10216E+06 | 17497 | 0.755386 | 99.2446 |
| 03:40 | 3.11966E+06 | 3.11525E+06 | 4407 | 0.103409 | 99.8966 |
| 03:50 | 3.11966E+06 | 3.11236E+06 | 7298 | 0.269058 | 99.7309 |
| 04:00 | 3.11966E+06 | 3.10457E+06 | 15096 | 0.681755 | 99.3182 |
| 04:10 | 3.11966E+06 | 3.07766E+06 | 41998 | 1.76721 | 98.2328 |
| 04:20 | 3.11966E+06 | 3.07535E+06 | 44310 | 1.9564 | 98.0436 |
| 04:30 | 3.11966E+06 | 3.05768E+06 | 61979 | 2.50758 | 97.4924 |
| 04:40 | 3.11966E+06 | 3.02762E+06 | 92041 | 3.80534 | 96.1947 |
| 04:50 | 3.11966E+06 | 3.00464E+06 | 115018 | 3.81686 | 96.1831 |
| 05:00 | 3.11966E+06 | 2.95627E+06 | 163393 | 4.54576 | 95.4542 |
| 05:10 | 3.11966E+06 | 2.93997E+06 | 179691 | 4.54385 | 95.4562 |
| 05:20 | 3.11966E+06 | 2.86657E+06 | 253088 | 6.77918 | 93.2208 |
| 05:30 | 3.11966E+06 | 2.81979E+06 | 299871 | 8.5307 | 91.4693 |
| 05:40 | 3.11966E+06 | 2.84113E+06 | 278536 | 7.66619 | 92.3338 |
| 05:50 | 3.11966E+06 | 2.79118E+06 | 328478 | 9.60967 | 90.3903 |
| 06:00 | 3.11966E+06 | 2.73031E+06 | 389355 | 12.401 | 87.599 |
| 06:10 | 3.11966E+06 | 2.74434E+06 | 375325 | 12.08 | 87.92 |
| 06:20 | 3.11966E+06 | 2.74145E+06 | 378216 | 12.3794 | 87.6206 |
| 06:30 | 3.11966E+06 | 2.77861E+06 | 341055 | 11.1176 | 88.8824 |
| 06:40 | 3.11966E+06 | 2.74919E+06 | 370468 | 12.6114 | 87.3886 |
| 06:50 | 3.11966E+06 | 2.67428E+06 | 445385 | 15.5734 | 84.4266 |
| 07:00 | 3.11966E+06 | 2.70008E+06 | 419586 | 14.196 | 85.804 |
| 07:10 | 3.11966E+06 | 2.73748E+06 | 382182 | 12.5636 | 87.4365 |
| 07:20 | 3.11966E+06 | 2.7225E+06 | 397159 | 13.5014 | 86.4986 |
| 07:30 | 3.11966E+06 | 2.72079E+06 | 398875 | 13.1071 | 86.893 |
C4
Well tracking video
Well images over time
Well over time:
| Time Point (hh:mm) | Total Area (µm²) | Total Covered Area (µm²) | Total Open Area (µm²) | Scratch Open Area (%) | Scratch Covered Area (%) |
| 00:00 | 3.11966E+06 | 711736 | 2.40793E+06 | 100 | 0 |
| 00:10 | 3.11966E+06 | 1.01369E+06 | 2.10597E+06 | 87.4041 | 12.5959 |
| 00:20 | 3.11966E+06 | 1.24645E+06 | 1.87321E+06 | 77.7133 | 22.2867 |
| 00:30 | 3.11966E+06 | 1.45982E+06 | 1.65984E+06 | 68.8488 | 31.1512 |
| 00:40 | 3.11966E+06 | 1.67702E+06 | 1.44265E+06 | 59.8354 | 40.1646 |
| 00:50 | 3.11966E+06 | 1.8833E+06 | 1.23637E+06 | 51.3139 | 48.6861 |
| 01:00 | 3.11966E+06 | 2.08144E+06 | 1.03822E+06 | 42.9727 | 57.0273 |
| 01:10 | 3.11966E+06 | 2.26016E+06 | 859498 | 35.4547 | 64.5453 |
| 01:20 | 3.11966E+06 | 2.42135E+06 | 698313 | 28.679 | 71.321 |
| 01:30 | 3.11966E+06 | 2.57534E+06 | 544321 | 22.1855 | 77.8145 |
| 01:40 | 3.11966E+06 | 2.76112E+06 | 358543 | 14.4132 | 85.5868 |
| 01:50 | 3.11966E+06 | 2.91388E+06 | 205783 | 8.18811 | 91.8119 |
| 02:00 | 3.11966E+06 | 3.02448E+06 | 95186 | 3.92452 | 96.0755 |
| 02:10 | 3.11966E+06 | 3.08231E+06 | 37351 | 1.5361 | 98.4639 |
| 02:20 | 3.11966E+06 | 3.07585E+06 | 43808 | 0.530319 | 99.4697 |
| 02:30 | 3.11966E+06 | 3.1024E+06 | 17261 | 0.65712 | 99.3429 |
| 02:40 | 3.11966E+06 | 3.09453E+06 | 25130 | 1.03315 | 98.9669 |
| 02:50 | 3.11966E+06 | 3.08731E+06 | 32352 | 1.00956 | 98.9904 |
| 03:00 | 3.11966E+06 | 3.08151E+06 | 38153 | 1.20173 | 98.7983 |
| 03:10 | 3.11966E+06 | 3.05954E+06 | 60117 | 2.00559 | 97.9944 |
| 03:20 | 3.11966E+06 | 2.90395E+06 | 215712 | 8.4055 | 91.5945 |
| 03:30 | 3.11966E+06 | 3.07179E+06 | 47872 | 1.37376 | 98.6262 |
| 03:40 | 3.11966E+06 | 3.05767E+06 | 61988 | 1.90994 | 98.0901 |
| 03:50 | 3.11966E+06 | 2.99348E+06 | 126179 | 4.59469 | 95.4053 |
| 04:00 | 3.11966E+06 | 3.07172E+06 | 47944 | 1.42094 | 98.5791 |
| 04:10 | 3.11966E+06 | 3.05838E+06 | 61281 | 1.80685 | 98.1932 |
| 04:20 | 3.11966E+06 | 2.97772E+06 | 141942 | 5.0235 | 94.9765 |
| 04:30 | 3.11966E+06 | 2.95719E+06 | 162475 | 5.4909 | 94.5091 |
| 04:40 | 3.11966E+06 | 2.99173E+06 | 127936 | 4.00127 | 95.9987 |
| 04:50 | 3.11966E+06 | 3.0316E+06 | 88063 | 2.44424 | 97.5558 |
| 05:00 | 3.11966E+06 | 2.99353E+06 | 126128 | 3.7655 | 96.2345 |
| 05:10 | 3.11966E+06 | 2.98561E+06 | 134054 | 3.71262 | 96.2874 |
| 05:20 | 3.11966E+06 | 2.9679E+06 | 151765 | 4.62463 | 95.3754 |
| 05:30 | 3.11966E+06 | 2.95007E+06 | 169593 | 4.78478 | 95.2152 |
| 05:40 | 3.11966E+06 | 2.9415E+06 | 178164 | 4.61558 | 95.3844 |
| 05:50 | 3.11966E+06 | 2.96771E+06 | 151948 | 3.84495 | 96.1551 |
| 06:00 | 3.11966E+06 | 2.95724E+06 | 162424 | 4.31924 | 95.6808 |
| 06:10 | 3.11966E+06 | 2.94333E+06 | 176331 | 4.31168 | 95.6883 |
| 06:20 | 3.11966E+06 | 2.94294E+06 | 176726 | 4.70291 | 95.2971 |
| 06:30 | 3.11966E+06 | 2.86832E+06 | 251338 | 6.53568 | 93.4643 |
| 06:40 | 3.11966E+06 | 2.8617E+06 | 257966 | 6.61322 | 93.3868 |
| 06:50 | 3.11966E+06 | 2.87379E+06 | 245875 | 6.11118 | 93.8888 |
| 07:00 | 3.11966E+06 | 2.9344E+06 | 185266 | 5.23402 | 94.766 |
| 07:10 | 3.11966E+06 | 2.84716E+06 | 272501 | 7.52215 | 92.4779 |
| 07:20 | 3.11966E+06 | 2.93376E+06 | 185903 | 5.61434 | 94.3857 |
| 07:30 | 3.11966E+06 | 2.96208E+06 | 157577 | 4.63871 | 95.3613 |
| 07:40 | 3.11966E+06 | 2.98912E+06 | 130542 | 3.5565 | 96.4435 |
| 07:50 | 3.11966E+06 | 2.93253E+06 | 187130 | 5.32988 | 94.6701 |
C5
Well tracking video
Well images over time
Well over time:
| Time Point (hh:mm) | Total Area (µm²) | Total Covered Area (µm²) | Total Open Area (µm²) | Scratch Open Area (%) | Scratch Covered Area (%) |
| 00:00 | 1.32738E+06 | 899591 | 427784 | 100 | 0 |
| 00:10 | 1.32738E+06 | 967000 | 360375 | 79.145 | 20.855 |
| 00:20 | 1.32738E+06 | 1.0305E+06 | 296874 | 64.4821 | 35.518 |
| 00:30 | 1.32738E+06 | 1.09757E+06 | 229803 | 50.4625 | 49.5376 |
| 00:40 | 1.32738E+06 | 1.151E+06 | 176374 | 37.3423 | 62.6577 |
| 00:50 | 1.32738E+06 | 1.20018E+06 | 127196 | 26.6347 | 73.3653 |
| 01:00 | 1.32738E+06 | 1.24867E+06 | 78707 | 14.785 | 85.215 |
| 01:10 | 1.32738E+06 | 1.28384E+06 | 43537 | 6.47289 | 93.5271 |
| 01:20 | 1.32738E+06 | 1.28875E+06 | 38624 | 5.75874 | 94.2413 |
| 01:30 | 1.32738E+06 | 1.30066E+06 | 26719 | 3.42442 | 96.5756 |
| 01:40 | 1.32738E+06 | 1.31735E+06 | 10023 | 1.09931 | 98.9007 |
| 01:50 | 1.32738E+06 | 1.314E+06 | 13372 | 1.02119 | 98.9788 |
| 02:00 | 1.32738E+06 | 1.31148E+06 | 15892 | 0.676155 | 99.3238 |
| 02:10 | 1.32738E+06 | 1.31207E+06 | 15302 | 0.499619 | 99.5004 |
| 02:20 | 1.32738E+06 | 1.31707E+06 | 10304 | 0.677567 | 99.3224 |
| 02:30 | 1.32738E+06 | 1.32007E+06 | 7306 | 0.277306 | 99.7227 |
| 02:40 | 1.32738E+06 | 1.31814E+06 | 9237 | 0.898232 | 99.1018 |
| 02:50 | 1.32738E+06 | 1.32157E+06 | 5805 | 0.254883 | 99.7451 |
| 03:00 | 1.32738E+06 | 1.32085E+06 | 6522 | 0.156235 | 99.8438 |
| 03:10 | 1.32738E+06 | 1.32391E+06 | 3461 | 0.000236511 | 99.9998 |
| 03:20 | 1.32738E+06 | 1.32588E+06 | 1494 | 0.144432 | 99.8556 |
| 03:30 | 1.32738E+06 | 1.32372E+06 | 3654 | 0.258423 | 99.7416 |
| 03:40 | 1.32738E+06 | 1.32367E+06 | 3703 | 0.556969 | 99.443 |
| 03:50 | 1.32738E+06 | 1.32578E+06 | 1599 | 0.0507431 | 99.9493 |
| 04:00 | 1.32738E+06 | 1.32488E+06 | 2496 | 0.327812 | 99.6722 |
| 04:10 | 1.32738E+06 | 1.32609E+06 | 1286 | 0.0472031 | 99.9528 |
| 04:20 | 1.32738E+06 | 1.32429E+06 | 3088 | 0.252762 | 99.7472 |
| 04:30 | 1.32738E+06 | 1.32226E+06 | 5114 | 0.261726 | 99.7383 |
| 04:40 | 1.32738E+06 | 1.32634E+06 | 1035 | 0.00896454 | 99.991 |
| 04:50 | 1.32738E+06 | 1.32497E+06 | 2406 | 0.129097 | 99.8709 |
| 05:00 | 1.32738E+06 | 1.32704E+06 | 339 | 0 | 100 |
| 05:10 | 1.32738E+06 | 1.32467E+06 | 2708 | 0.607948 | 99.3921 |
| 05:20 | 1.32738E+06 | 1.32459E+06 | 2789 | 0.513542 | 99.4865 |
| 05:30 | 1.32738E+06 | 1.32634E+06 | 1034 | 0.187859 | 99.8121 |
| 05:40 | 1.32738E+06 | 1.32072E+06 | 6655 | 0.736572 | 99.2634 |
| 05:50 | 1.32738E+06 | 1.3261E+06 | 1276 | 0.0226593 | 99.9773 |
| 06:00 | 1.32738E+06 | 1.3262E+06 | 1178 | 0.230339 | 99.7697 |
| 06:10 | 1.32738E+06 | 1.32501E+06 | 2367 | 0.0280838 | 99.9719 |
| 06:20 | 1.32738E+06 | 1.32671E+06 | 667 | 0.0292664 | 99.9707 |
| 06:30 | 1.32738E+06 | 1.32605E+06 | 1323 | 0.00212097 | 99.9979 |
| 06:40 | 1.32738E+06 | 1.32536E+06 | 2017 | 0.429527 | 99.5705 |
| 06:50 | 1.32738E+06 | 1.32656E+06 | 814 | 0.0545197 | 99.9455 |
| 07:00 | 1.32738E+06 | 1.32717E+06 | 203 | 0.0162811 | 99.9837 |
| 07:10 | 1.32738E+06 | 1.32522E+06 | 2159 | 0.25441 | 99.7456 |
| 07:20 | 1.32738E+06 | 1.32524E+06 | 2132 | 0.24025 | 99.7598 |
| 07:30 | 1.32738E+06 | 1.32556E+06 | 1813 | 0.230812 | 99.7692 |
| 07:40 | 1.32738E+06 | 1.32706E+06 | 312 | 0.0589981 | 99.941 |
| 07:50 | 1.32738E+06 | 1.3262E+06 | 1172 | 0.00589752 | 99.9941 |
| 08:00 | 1.32738E+06 | 1.32421E+06 | 3168 | 0.00802612 | 99.992 |
| 08:10 | 1.32738E+06 | 1.32682E+06 | 556 | 0.0191193 | 99.9809 |
| 08:20 | 1.32738E+06 | 1.32671E+06 | 664 | 0 | 100 |
| 08:30 | 1.32738E+06 | 1.3263E+06 | 1073 | 0.185501 | 99.8145 |
| 08:40 | 1.32738E+06 | 1.32636E+06 | 1019 | 0.000236511 | 99.9998 |
| 08:50 | 1.32738E+06 | 1.32563E+06 | 1742 | 0.0653763 | 99.9346 |
| 09:00 | 1.32738E+06 | 1.32694E+06 | 435 | 0.043663 | 99.9563 |
| 09:10 | 1.32738E+06 | 1.32546E+06 | 1919 | 0.0948715 | 99.9051 |
| 09:20 | 1.32738E+06 | 1.32698E+06 | 391 | 0.0151062 | 99.9849 |
| 09:30 | 1.32738E+06 | 1.32678E+06 | 597 | 0.0745773 | 99.9254 |
| 09:40 | 1.32738E+06 | 1.31943E+06 | 7945 | 0.836403 | 99.1636 |
| 09:50 | 1.32738E+06 | 1.32631E+06 | 1065 | 0 | 100 |
| 10:00 | 1.32738E+06 | 1.3265E+06 | 876 | 0.12178 | 99.8782 |
| 10:10 | 1.32738E+06 | 1.32684E+06 | 535 | 0.00141907 | 99.9986 |
| 10:20 | 1.32738E+06 | 1.32621E+06 | 1162 | 0 | 100 |
| 10:30 | 1.32738E+06 | 1.32704E+06 | 333 | 0.0233612 | 99.9766 |
| 10:40 | 1.32738E+06 | 1.32735E+06 | 30 | 0 | 100 |
| 10:50 | 1.32738E+06 | 1.32593E+06 | 1445 | 0.00448608 | 99.9955 |
| 11:00 | 1.32738E+06 | 1.32707E+06 | 302 | 0 | 100 |
| 11:10 | 1.32738E+06 | 1.32227E+06 | 5103 | 0.238602 | 99.7614 |
| 11:20 | 1.32738E+06 | 1.32573E+06 | 1649 | 0.0191193 | 99.9809 |
| 11:30 | 1.32738E+06 | 1.32554E+06 | 1835 | 0.202255 | 99.7977 |
| 11:40 | 1.32738E+06 | 1.32734E+06 | 32 | 0 | 100 |
| 11:50 | 1.32738E+06 | 1.32641E+06 | 968 | 0.15081 | 99.8492 |
C6
Well tracking video
Well images over time
Well over time:
| Time Point (hh:mm) | Total Area (µm²) | Total Covered Area (µm²) | Total Open Area (µm²) | Scratch Open Area (%) | Scratch Covered Area (%) |
| 00:00 | 1.32738E+06 | 489085 | 838290 | 100 | 0 |
| 00:10 | 1.32738E+06 | 507140 | 820235 | 97.6715 | 2.32854 |
| 00:20 | 1.32738E+06 | 520210 | 807165 | 96.1611 | 3.83886 |
| 00:30 | 1.32738E+06 | 534769 | 792606 | 94.4587 | 5.54129 |
| 00:40 | 1.32738E+06 | 550470 | 776905 | 92.5815 | 7.41854 |
| 00:50 | 1.32738E+06 | 564013 | 763362 | 90.9455 | 9.0545 |
| 01:00 | 1.32738E+06 | 581793 | 745582 | 88.7903 | 11.2097 |
| 01:10 | 1.32738E+06 | 599883 | 727492 | 86.5005 | 13.4995 |
| 01:20 | 1.32738E+06 | 618264 | 709111 | 84.4767 | 15.5233 |
| 01:30 | 1.32738E+06 | 631571 | 695804 | 82.7406 | 17.2594 |
| 01:40 | 1.32738E+06 | 645303 | 682072 | 81.0546 | 18.9454 |
| 01:50 | 1.32738E+06 | 656063 | 671312 | 79.5833 | 20.4167 |
| 02:00 | 1.32738E+06 | 673184 | 654191 | 77.4699 | 22.5301 |
| 02:10 | 1.32738E+06 | 685276 | 642099 | 76.0483 | 23.9517 |
| 02:20 | 1.32738E+06 | 700988 | 626387 | 74.0663 | 25.9337 |
| 02:30 | 1.32738E+06 | 717005 | 610370 | 72.3773 | 27.6227 |
| 02:40 | 1.32738E+06 | 737188 | 590187 | 70.3269 | 29.6731 |
| 02:50 | 1.32738E+06 | 747663 | 579712 | 68.3919 | 31.6081 |
| 03:00 | 1.32738E+06 | 766503 | 560872 | 66.6712 | 33.3288 |
| 03:10 | 1.32738E+06 | 749273 | 578102 | 65.8524 | 34.1476 |
| 03:20 | 1.32738E+06 | 756248 | 571127 | 64.3854 | 35.6146 |
| 03:30 | 1.32738E+06 | 798870 | 528505 | 62.1924 | 37.8076 |
| 03:40 | 1.32738E+06 | 810857 | 516518 | 60.0277 | 39.9723 |
| 03:50 | 1.32738E+06 | 732581 | 594794 | 58.6396 | 41.3604 |
| 04:00 | 1.32738E+06 | 645389 | 681986 | 58.8342 | 41.1658 |
| 04:10 | 1.32738E+06 | 874495 | 452880 | 53.591 | 46.409 |
| 04:20 | 1.32738E+06 | 765424 | 561951 | 53.6619 | 46.3381 |
| 04:30 | 1.32738E+06 | 692380 | 634995 | 54.5916 | 45.4084 |
| 04:40 | 1.32738E+06 | 887967 | 439408 | 47.7445 | 52.2555 |
| 04:50 | 1.32738E+06 | 741187 | 586188 | 49.9239 | 50.0761 |
| 05:00 | 1.32738E+06 | 910129 | 417246 | 42.97 | 57.03 |
| 05:10 | 1.32738E+06 | 946474 | 380901 | 40.3657 | 59.6343 |
| 05:20 | 1.32738E+06 | 944226 | 383149 | 39.468 | 60.532 |
| 05:30 | 1.32738E+06 | 979519 | 347856 | 36.617 | 63.383 |
| 05:40 | 1.32738E+06 | 1.04339E+06 | 283986 | 33.7809 | 66.2191 |
| 05:50 | 1.32738E+06 | 1.05131E+06 | 276068 | 31.4185 | 68.5815 |
| 06:00 | 1.32738E+06 | 1.02594E+06 | 301431 | 30.1367 | 69.8634 |
| 06:10 | 1.32738E+06 | 1.00522E+06 | 322154 | 33.2574 | 66.7426 |
| 06:20 | 1.32738E+06 | 966826 | 360549 | 30.0936 | 69.9064 |
| 06:30 | 1.32738E+06 | 1.11685E+06 | 210525 | 24.3249 | 75.6752 |
| 06:40 | 1.32738E+06 | 1.12565E+06 | 201721 | 22.3296 | 77.6704 |
| 06:50 | 1.32738E+06 | 1.10681E+06 | 220566 | 20.1969 | 79.8031 |
| 07:00 | 1.32738E+06 | 1.1727E+06 | 154679 | 17.6547 | 82.3453 |
| 07:10 | 1.32738E+06 | 1.18739E+06 | 139990 | 15.754 | 84.246 |
| 07:20 | 1.32738E+06 | 1.19577E+06 | 131606 | 14.5382 | 85.4618 |
| 07:30 | 1.32738E+06 | 1.15859E+06 | 168782 | 12.4519 | 87.5481 |
| 07:40 | 1.32738E+06 | 1.22682E+06 | 100555 | 11.2963 | 88.7037 |
| 07:50 | 1.32738E+06 | 1.23204E+06 | 95331 | 9.83872 | 90.1613 |
| 08:00 | 1.32738E+06 | 1.19896E+06 | 128414 | 10.8665 | 89.1335 |
| 08:10 | 1.32738E+06 | 1.25218E+06 | 75193 | 8.40405 | 91.596 |
| 08:20 | 1.32738E+06 | 1.21823E+06 | 109147 | 8.9279 | 91.0721 |
| 08:30 | 1.32738E+06 | 1.27232E+06 | 55053 | 6.27026 | 93.7297 |
| 08:40 | 1.32738E+06 | 1.23197E+06 | 95403 | 7.31663 | 92.6834 |
| 08:50 | 1.32738E+06 | 1.12067E+06 | 206709 | 15.494 | 84.506 |
| 09:00 | 1.32738E+06 | 1.19802E+06 | 129360 | 9.87701 | 90.123 |
| 09:10 | 1.32738E+06 | 1.15803E+06 | 169348 | 13.3279 | 86.6721 |
| 09:20 | 1.32738E+06 | 1.28189E+06 | 45490 | 5.04847 | 94.9515 |
| 09:30 | 1.32738E+06 | 1.20695E+06 | 120429 | 12.5368 | 87.4632 |
| 09:40 | 1.32738E+06 | 1.24491E+06 | 82469 | 5.4296 | 94.5704 |
| 09:50 | 1.32738E+06 | 1.26479E+06 | 62581 | 5.6321 | 94.3679 |
| 10:00 | 1.32738E+06 | 1.30251E+06 | 24867 | 2.93973 | 97.0603 |
| 10:10 | 1.32738E+06 | 1.26192E+06 | 65454 | 6.96283 | 93.0372 |
| 10:20 | 1.32738E+06 | 1.2097E+06 | 117678 | 11.4847 | 88.5153 |
| 10:30 | 1.32738E+06 | 1.22995E+06 | 97424 | 9.91689 | 90.0831 |
| 10:40 | 1.32738E+06 | 1.2009E+06 | 126474 | 11.4056 | 88.5944 |
| 10:50 | 1.32738E+06 | 1.04494E+06 | 282434 | 24.9044 | 75.0956 |
| 11:00 | 1.32738E+06 | 1.22503E+06 | 102341 | 11.9809 | 88.0191 |
| 11:10 | 1.32738E+06 | 1.25231E+06 | 75061 | 8.48842 | 91.5116 |
| 11:20 | 1.32738E+06 | 1.25513E+06 | 72242 | 8.47828 | 91.5217 |
| 11:30 | 1.32738E+06 | 1.26535E+06 | 62025 | 4.45805 | 95.542 |
| 11:40 | 1.32738E+06 | 1.20954E+06 | 117831 | 9.36857 | 90.6314 |
| 11:50 | 1.32738E+06 | 1.2059E+06 | 121476 | 12.0179 | 87.9821 |
C7
Well tracking video
Well images over time
Well over time:
| Time Point (hh:mm) | Total Area (µm²) | Total Covered Area (µm²) | Total Open Area (µm²) | Scratch Open Area (%) | Scratch Covered Area (%) |
| 00:00 | 1.32738E+06 | 718192 | 609183 | 100 | 0 |
| 00:10 | 1.32738E+06 | 743498 | 583877 | 92.9936 | 7.00645 |
| 00:20 | 1.32738E+06 | 756447 | 570928 | 89.9808 | 10.0192 |
| 00:30 | 1.32738E+06 | 768886 | 558489 | 87.4017 | 12.5983 |
| 00:40 | 1.32738E+06 | 768931 | 558444 | 86.6848 | 13.3152 |
| 00:50 | 1.32738E+06 | 787981 | 539394 | 84.0692 | 15.9308 |
| 01:00 | 1.32738E+06 | 805543 | 521832 | 81.1556 | 18.8444 |
| 01:10 | 1.32738E+06 | 811995 | 515380 | 79.9353 | 20.0647 |
| 01:20 | 1.32738E+06 | 821520 | 505855 | 78.7116 | 21.2884 |
| 01:30 | 1.32738E+06 | 836375 | 491000 | 76.6653 | 23.3347 |
| 01:40 | 1.32738E+06 | 842682 | 484693 | 75.6962 | 24.3038 |
| 01:50 | 1.32738E+06 | 853714 | 473661 | 73.9461 | 26.0539 |
| 02:00 | 1.32738E+06 | 865545 | 461830 | 72.0349 | 27.9651 |
| 02:10 | 1.32738E+06 | 874950 | 452425 | 70.4308 | 29.5692 |
| 02:20 | 1.32738E+06 | 887582 | 439793 | 68.3361 | 31.6639 |
| 02:30 | 1.32738E+06 | 892839 | 434536 | 67.243 | 32.7571 |
| 02:40 | 1.32738E+06 | 907710 | 419665 | 65.2684 | 34.7316 |
| 02:50 | 1.32738E+06 | 915869 | 411506 | 63.8483 | 36.1517 |
| 03:00 | 1.32738E+06 | 918220 | 409155 | 63.4115 | 36.5885 |
| 03:10 | 1.32738E+06 | 924466 | 402909 | 62.3799 | 37.6201 |
| 03:20 | 1.32738E+06 | 933974 | 393401 | 60.9221 | 39.0779 |
| 03:30 | 1.32738E+06 | 936315 | 391060 | 60.2788 | 39.7212 |
| 03:40 | 1.32738E+06 | 946122 | 381253 | 58.7854 | 41.2146 |
| 03:50 | 1.32738E+06 | 954662 | 372713 | 57.5183 | 42.4817 |
| 04:00 | 1.32738E+06 | 969331 | 358044 | 55.4556 | 44.5444 |
| 04:10 | 1.32738E+06 | 983372 | 344003 | 53.5379 | 46.4621 |
| 04:20 | 1.32738E+06 | 984898 | 342477 | 53.3729 | 46.6271 |
| 04:30 | 1.32738E+06 | 995176 | 332199 | 51.6064 | 48.3937 |
| 04:40 | 1.32738E+06 | 1.0056E+06 | 321776 | 50.0034 | 49.9966 |
| 04:50 | 1.32738E+06 | 1.01801E+06 | 309369 | 48.2898 | 51.7102 |
| 05:00 | 1.32738E+06 | 1.0251E+06 | 302273 | 43.6461 | 56.3539 |
| 05:10 | 1.32738E+06 | 1.03136E+06 | 296020 | 38.851 | 61.149 |
| 05:20 | 1.32738E+06 | 1.04109E+06 | 286290 | 36.2928 | 63.7072 |
| 05:30 | 1.32738E+06 | 1.0417E+06 | 285672 | 35.4088 | 64.5912 |
| 05:40 | 1.32738E+06 | 1.04967E+06 | 277710 | 33.7414 | 66.2586 |
| 05:50 | 1.32738E+06 | 1.05238E+06 | 274991 | 33.009 | 66.991 |
| 06:00 | 1.32738E+06 | 1.05793E+06 | 269441 | 31.8495 | 68.1505 |
| 06:10 | 1.32738E+06 | 1.0642E+06 | 263179 | 30.7534 | 69.2466 |
| 06:20 | 1.32738E+06 | 1.06785E+06 | 259525 | 30.5747 | 69.4253 |
| 06:30 | 1.32738E+06 | 1.08099E+06 | 246388 | 29.2872 | 70.7129 |
| 06:40 | 1.32738E+06 | 1.07965E+06 | 247724 | 27.9843 | 72.0157 |
| 06:50 | 1.32738E+06 | 1.08593E+06 | 241445 | 29.1766 | 70.8234 |
| 07:00 | 1.32738E+06 | 1.08295E+06 | 244429 | 27.3602 | 72.6399 |
| 07:10 | 1.32738E+06 | 1.08613E+06 | 241249 | 28.7563 | 71.2437 |
| 07:20 | 1.32738E+06 | 1.08905E+06 | 238324 | 28.631 | 71.369 |
| 07:30 | 1.32738E+06 | 1.09033E+06 | 237043 | 28.5917 | 71.4083 |
| 07:40 | 1.32738E+06 | 1.09381E+06 | 233562 | 27.9871 | 72.0129 |
| 07:50 | 1.32738E+06 | 1.09847E+06 | 228901 | 27.4064 | 72.5936 |
| 08:00 | 1.32738E+06 | 1.10407E+06 | 223302 | 26.9925 | 73.0075 |
| 08:10 | 1.32738E+06 | 1.09999E+06 | 227382 | 27.5394 | 72.4606 |
| 08:20 | 1.32738E+06 | 1.10541E+06 | 221966 | 27.1179 | 72.8821 |
| 08:30 | 1.32738E+06 | 1.11686E+06 | 210519 | 25.7179 | 74.2822 |
| 08:40 | 1.32738E+06 | 1.12344E+06 | 203932 | 25.1276 | 74.8724 |
| 08:50 | 1.32738E+06 | 1.12725E+06 | 200127 | 24.6433 | 75.3567 |
| 09:00 | 1.32738E+06 | 1.1281E+06 | 199271 | 24.5018 | 75.4983 |
| 09:10 | 1.32738E+06 | 1.12867E+06 | 198704 | 24.4201 | 75.5799 |
| 09:20 | 1.32738E+06 | 1.13595E+06 | 191426 | 23.9437 | 76.0563 |
| 09:30 | 1.32738E+06 | 1.14249E+06 | 184884 | 23.3698 | 76.6303 |
| 09:40 | 1.32738E+06 | 1.1438E+06 | 183577 | 22.8672 | 77.1328 |
| 09:50 | 1.32738E+06 | 1.14631E+06 | 181063 | 22.2333 | 77.7667 |
| 10:00 | 1.32738E+06 | 1.15506E+06 | 172318 | 21.5956 | 78.4044 |
| 10:10 | 1.32738E+06 | 1.15705E+06 | 170326 | 21.7865 | 78.2135 |
| 10:20 | 1.32738E+06 | 1.15673E+06 | 170646 | 21.8035 | 78.1965 |
| 10:30 | 1.32738E+06 | 1.16242E+06 | 164953 | 21.0564 | 78.9436 |
| 10:40 | 1.32738E+06 | 1.17039E+06 | 156981 | 20.6572 | 79.3429 |
| 10:50 | 1.32738E+06 | 1.16669E+06 | 160690 | 20.6491 | 79.3509 |
| 11:00 | 1.32738E+06 | 1.16999E+06 | 157385 | 20.0812 | 79.9188 |
| 11:10 | 1.32738E+06 | 1.17291E+06 | 154470 | 19.7448 | 80.2552 |
| 11:20 | 1.32738E+06 | 1.17839E+06 | 148984 | 19.0854 | 80.9146 |
| 11:30 | 1.32738E+06 | 1.17711E+06 | 150265 | 19.5324 | 80.4676 |
| 11:40 | 1.32738E+06 | 1.181E+06 | 146376 | 18.6981 | 81.3019 |
| 11:50 | 1.32738E+06 | 1.19031E+06 | 137061 | 17.7693 | 82.2307 |