How to Purchase
IO assays are only avaible via consulation. Please contact us directly for a non-confidential presentation and discussion of your requirements.
After a consulation, purchasing our services is as easy as click of a mouse when you use Scientist.com or Science-Exchange.
The MetaVi Lab's killing efficiency assay is designed to help you answer questions such as:
- How to measure the overall killing effectiveness of an IO treatment?
- How many engineered immune cells are required to eliminate a cancer cell population that itself is rapidly expanding?
- How many target cells can one immune cell kill?
- What percentage of the immune cells are actively engaging with target cells?
- How long does it take to kill one cancer cell?
- Do the immune cells get exhausted and then recover?
- Do the immune cells die after killing target cells?
- How do the immune cells discover the target cells?
With no fluorescent labels, using only morphology in phase-contrast or brightfield, FastTrack AI can track and count living and dead individual cells over time. This dramatically increases the accuracy of cell death measurements.
With the addition of a fluorescent label on one cell type, FastTrack AI can track, and identify individual target and effector cells over time. This allows the precise measurement of serial killing efficiency by tracking thousands of individual cells.
With the usage of ibidi patterned adhension and micro-well slides, we offer the additional advantage of isolating attacks and dramatically increased precision in serial killing measurements.
What are we offering?
We run an internet hosted software service (on Amazon Web Services). For high-throughput customers we install a special computer on your site that interfaces with your microscope data system and automates the uploading of images into our webservice.
We also customize our analysis per colloboration with our customers and offer consulation services for the duration of the service contract.
Our automated reports generate over 50 metrics. We sample just a few below. All data is avaible in easy to read charts and also raw excel data.
Demonstrating Killing Efficiency Movie with ibidi Patterned SlideThis is a demonstration of FastTrack AI measuring T cell killing efficiency on the ibidi adhesion pad grid slide. The source movie is phase contrast with no dyes or labels. The AI system tags the imune cells with a blue mark. The AI system tags the cancer cells with a green number. Cells which transition from living to dead are coded in red. Notice dead cell matter is not interpreted as living cells.
Cytotoxicity over Time
Histogram of Synapse Time to Cell Death
Average Number of Pathogens killed per T cell
Histogram of Percentage of T cells by Number of Effective Attacks
Learn more about MetaVi Labs Killing Efficiency AssaysKilling Efficiency On-Line Documentation
Learn more about ibidi Micro-patterningMicro-patterning
Killing Assay PosterA Micropatterning-Supported High Throughput CAR-T Cell Potency Assay With Single Cell Resolution (PDF)
Presented at the ASCB|EMBO Meeting 2019, Washington DC, USA.